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. Author manuscript; available in PMC: 2023 Jun 2.
Published in final edited form as: Mol Cell. 2022 May 20;82(11):2098–2112.e4. doi: 10.1016/j.molcel.2022.04.026

Figure 5. The Arp5 module interacts with the acidic patch to regulate INO80 sliding.

Figure 5.

(A) Model of the nucleosome (PDB: 1KX5) from different angles with location of the engineered PstI restriction site in green. Proximal H2A-H2B dimer is in cyan.

(B) Rate constants of cutting by PstI for WT and double APM 601 + 40 N, with saturating ATP and saturating WT INO80 (coral) or INO80(Δarp5) (blue).

(C) Example native gels showing remodeling of WT (top panel) and double APM (bottom panel) 601 + 80 N with WT INO80 (coral) and INO80(Δarp5) (blue).

(D) Quantification of rate constants from multiple repeats of data, such as that shown in (C).

(E) Rate constants for INO80 ATPase activity with WT and double APM, 601 + 80 N. All ATPase assays were performed under the same conditions as native gel remodeling.

(F) Observed rate constants for ATPase activity of WT INO80 (coral) and INO80(Δarp5) (blue) on 601 + 40 N, 601 + 60 N, and 601 + 80 N. Error bars represent SEM from n > 3.