Figure 1:

Comparison of the yield and integrity of DNA extracted with commercially available kits. (A) A representative image of the hematoxylin–eosin stained tissue section used for the comparison. (B) The mean yield of DNA extracted from FFPE sections using DNeasy Blood and Tissue kit (DNeasy) from Qiagen; Quick-DNA FFPE Miniprep kit (Quick) from Zymo Research; NucleoSpin DNA FFPE XS kit (NucleoSpin) from MACHEREY-NAGE; and Agencourt FormaPure XL DNA kit (Agencourt) from Beckman Coulter. (C) Image showing agarose gel electrophoresis of the extracted DNA the yields of which are presented in B. Note that the amount of DNA per well was adjusted for the visibility. (D and E) Comparison of DNA extracted from an FFPE tissue section and fresh frozen tissue. For both tissues, DNeasy Blood and Tissue kit was used. Image showing agarose gel electrophoresis of the extracted DNA (D). Comparison of qPCR amplifiable DNA fragments in the extracted DNA (E). The error bars in B and E were calculated from three independent experiments.