Figure 2:

Optimization of the conditions for DNA extraction from FFPE tissue sections. (A–C) Effect of temperature and duration of incubation on reverse-crosslinking of FFPE tissue sections. The qPCR-based quantitation of 200 bp fragment (A), DNA integrity index (B), and the agarose gel image (C) are shown. (D and E) The improved DNA integrity with increasing concentration of Tris agarose gel image of extracted DNA (D) and DNA integrity index (E) are shown. (F–H) Comparison of DNA yield (F), qPCR-based quantitation of various amplicons (G), and DNA yield per slice (H) for DNA extracted using HiTE and DNeasy. Asterisks indicate significant (P < 0.05) differences between the indicated datasets (F, G and H, paired t-test). The error bars in F–H were calculated from three independent experiments.