Figure 3:

Comparison of the yield and integrity of DNA extracted from human FFPE tissue sections subjected to storage for different periods. (A–C) The DNA yield per slice (A), representative images of DNA electrophoresed on agarose gels (B), and amount of 200 bp fragment in the DNA measured using a qPCR-based assay (C) compared vis-à-vis the storage period. (D and E) The yield of sequencing library (D) and fragment size of amplified libraries (E) compared for DNA extracted using DNeasy and HiTE. For FFPE-DNAs extracted from blocks stored for a period of 1 year or less, the library yields with sonicated DNA are also shown (marked with dashed line). (F) The calculated yield of sequence library per FFPE tissue section compared for DNA extracted using DNeasy and HiTE. Asterisks indicate significant (P < 0.05) differences between the indicated datasets (A, C, D and F, paired t-test). The error bars in A, C, D, and F were calculated from three independent experiments.