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. Author manuscript; available in PMC: 2022 Aug 4.
Published in final edited form as: J Vis Exp. 2020 Apr 10;(158):10.3791/60996. doi: 10.3791/60996

Figure 3: Drug treatment can reduce xenografted tumor area in vivo.

Figure 3:

Representative images of zebrafish injected in the pericardium or yolk after 3 days of treatment with DMSO or drugs, either vincristine or dexamethasone. Area of engrafted tumor mass was quantified by setting a fluorescence threshold using ImageJ, selecting all pixels above the set threshold, and measuring the area and mean fluorescence of the selected regions. Pixels above the selected threshold appear in black, while pixels below the threshold appear in white. Pixels were measured in both the yolk and pericardium injected zebrafish images (A). Treatment with vincristine led to a decrease in engrafted tumor area compared to DMSO control with n = 4 fish treated per group (B). SD = standard deviation. Scale bar = 250 μm.