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. Author manuscript; available in PMC: 2022 Aug 4.
Published in final edited form as: J Vis Exp. 2020 Apr 10;(158):10.3791/60996. doi: 10.3791/60996

Table of Materials.

Name of Material/Equipment Company Catalog Number Comments/Description
10× TBE Liquid Concentrate VWR 0658-5L
96-well plate, flat bottom CELLTREAT 229195 VAST is compatible with a variety of standard or deep well 24, 48, or 96 well plates
Agarose Fisher Scientific BP160-500
Borosilicate Glass Capillary without Filament Sutter Instrument Company B100-50-10
Dexamethasone Enzo Life Sciences BML-EI126-0001
DMSO Sigma-Aldrich D2438-5X10ML
E3 media N/A 5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl2, 0.33 mM MgSO4
Femtotips Microloader Tips Eppendorf 930001007
Fetal Bovine Serum (Premium Heat Inactivated) Atlanta Biologicals S11150H
ImageJ FIJI N/A https://imagej.net/Fiji
Iscove’s Modified Dulbecco’s Medium STEMCELL Technologies 36150
Large Particle (LP) Sampler Union Biometrica N/A http://www.unionbio.com/copas/features.aspx?id=8
Methotrexate Sigma-Aldrich A6770-10MG
Mineral Oil Fisher Scientific BP26291
Phosphate Buffered Saline (1×) Caisson labs PBL06-6X500ML
Stage Micrometer (400-Stage) Hausser Scientific 400-S
Tricaine-S Pentair Aquatic TRS1
Trypan Blue Thermo Fisher T10282
VAST Bioimager Union Biometrica N/A https://www.unionbio.com/vast/
Vincristine Sulfate Enzo Life Sciences BML-T117-0005
Vybrant DiI Stain Thermo Fisher V22885