Skip to main content
. 2022 Aug 3;42(31):6038–6052. doi: 10.1523/JNEUROSCI.0115-22.2022

Figure 5.

Figure 5.

Presynaptic t-LTP requires NMDA and Group I mGluR1 metabotropic glutamate receptors. A, Addition of d-AP5 (50 μm) or MK-801 (500 μm) to the superfusion solution prevented t-LTP induction. The EPSP slope is shown in d-AP5-treated (red squares), MK-801-treated (gray squares), and untreated cells (black triangles). The traces show the EPSP before (1) and 30 min after (2) pairing. B, Summary of the results. C, The t-LTP requires mGluR. The EPSP slopes monitored in control slices (black triangles) and in slices treated with the mGluR antagonist LY341495 (100 μm, red squares) or the mGluR1 antagonist LY367385 (100 μm, gray squares) are shown following postpairing before prepairing. The traces show the EPSP before (1) and 30 min after (2) pairing. D, Summary of the results. E, The t-LTP requires activation of postsynaptic mGluRs. Time course of t-LTP induction in control conditions (black triangles) and with the postsynaptic neuron (red squares) loaded with GDPβS (1 mm). Inset, Traces show the EPSP before (1) and 30 min after pairing (2) in control slices or when the postsynaptic neurons are loaded with GDPβS. F, Summary of the results. Error bars indicate SEM, and the number of slices is shown in parentheses. Kruskal–Wallis test (B, D) and Mann–Whitney U (F); *p < 0.05, **p < 0.01, ***p < 0.001.