Table 4.
The main difference of the pre-processing in the four pharmacopoeias.
| Pharmacopoeia Name | Extraction Method | Temperature | Heating Time | Reagent |
|---|---|---|---|---|
| CP | Trichloromethane soxhlet and butannol ultrasonic extraction |
60 °C | 3 h | Trichloromethane; Butanol |
| USP | Water bath reflux extraction | 100 °C | 1 h | Ethanol-water (4:6) |
| EP | Water bath reflux extraction | 100 °C | 1 h | Methanol-water (50:50); Acetonitrile-water (20:80) |
| J/KP | Shake extraction | Room temperature | - | Methanol-water (3:5); Dilute sodium hydroxide solution; 0.1 mol/L Hydrochloric acid solution |