FIGURE 3.

SREBP-1 is increased in the neointima following stent placement. A stent was deployed in rat abdominal aortas for 4 weeks. Subsequently, vessels were harvested and analyzed by immunohistochemistry and immunofluorescence. Six μm sections were stained as described below. A, Control normal rat abdominal aorta stained with H&E reveals no neointima but a medial and adventitial (top) layer. B, Immunohistochemical staining for SREBP-1 in normal control untreated rat abdominal aorta is undetectable. C, Following stent placement for 4 weeks, a neointima layer is now visible and robust staining of SREBP-1 is detected. D, Higher magnification of SREBP-1 staining within the neointima. E, Immunofluorescence of FITC-anti-αSMA reveals robust staining within the neointima. F, H&E staining of the abdominal aorta following 4 weeks of stent placement. Reprinted with permission from Zhou et al.²² Copyright American College of Cardiology Foundation. All permission requests for this image should be made to the copyright holder. FITC indicates fluorescein isothiocyanate, H&E, hematoxylin and eosin; NI, neointima; SREBP1, sterol regulatory element-binding protein 1; αSMA, α-smooth muscle actin.