FIGURE 4.

SREBP-1 is increased in both the neointima and VSMC layer of mouse carotid arteries following vascular injury using a model of angioplasty. Four weeks after probe injury, carotid arteries were harvested and subjected to immunofluorescence or immunohistochemistry as indicated. Sections of 6 μm were stained with anti-SREBP-1 or Cy3-anti-α-SMA or stained using H&E. Nuclei were stained with DAPI in blue. Merge image of SREBP-1 (green) and α-SMA (red) is shown in yellow. SREBP-1 detected using immunohistochemistry is represented in purple and indicated by the small arrow. Neointima is indicated as the area between arrowheads in the H&E-stained and immunohistochemistry sections. In uninjured arteries from the same animals, SREBP-1 was barely detected under the same conditions by both methods (data not shown). Reprinted with permission from Zhou et al.²² Copyright American College of Cardiology Foundation. All permission requests for this image should be made to the copyright holder. DAPI indicates 4',6-diamidino-2-phenylindole; H&E, hematoxylin and eosin; IH-SREBP-1, immunohistochemistry for SREBP-1; SREBP1, sterol regulatory element-binding protein 1; VSMC, vascular smooth muscle cell; αSMA, α-smooth muscle actin.