FIG 2. Hypoxia depletes CD45⁺ cells in BMSC cultures.

A) Phase contrast images of passage 0 BMSCs after 7 days in either 21% O₂ (normoxia, Nx) or 1% O₂ (hypoxia, Hx). Representative flow analysis plots and quantification of the percentage of CD45⁺, PDGFRα⁺ and PDGFRα⁺;tdTomato⁺ double positive cells in B) WT or C) LepRCre;Rosa26^tdTm/+. Each graph represents an individual trial using BMSCs isolated from 2–3M male mice were used for analysis, n≥3, unpaired two tailed t-tests, significance p < 0.05