Table 1.
Semipolar metabolites found in the methanolic extract of Polygala tenuifolia root measured by ultrahigh-performance liquid chromatography–tandem mass spectrometry.
| No. | Retention time (min) | MW | [M−H]− (m/z) | [2M−H]− (m/z) | Fragment ions (m/z) | Chemicals |
|---|---|---|---|---|---|---|
| 1 | 5.29 | 462 | 460.9 | 922.9 | 299.1, 136.9 | Sibiricose A3 |
| 2 | 7.5 | 518 | 516.7 | 1034.9 | 340.6, 192.8 | Arillalose B |
| 3 | 7.96 | 548 | 546.8 | 1094.8 | 222.6, 204.9 | Sibiricose A6/A1 |
| 4 | 10.11 | 548 | 546.9 | 1094.3 | 222.8, 204.8 | Sibiricose A6/A1 |
| 5 | 10.96 | 568 | 566.8 | 435.2, 314.5 | Polygalaxanthone III or VIII | |
| 6 | 11.73 | 538 | 536.7 | 386.7, 266.7 | Sibiricaxanthone B | |
| 7 | 12.75 | 568 | 566.5 | 434.8, 314.9 | Polygalaxanthone III or VIII | |
| 8 | 12.96 | 568 | 567.0 | 1134.5 | 435.1, 416.9, 344.8 | Polygalaxanthone XI |
| 9 | 13.16 | 562 | 560.7 | 1123.2 | 399.0, 341.0, 236.8 | Sibiricose A2 |
| 10 | 13.69 | 256 | 255.0 | 511.0 | 212.9, 168.5 | 1-Hydroxy-2,3-methylenedioxyxanthone |
| 11 | 13.77 | 550 | 548.8 | 467.3, 254.9 | — | |
| 12 | 14.3 | 668 | 666.7 | 1334.9 | 461.1, 280.8, 239.1 | Tenuifoliside B |
| 13 | 14.4 | 754 | 752.7 | 1506.7 | 705.2, 528.5, 205.0 | — |
| 14 | 16.3 | 582 | 580.9 | 549.0, 323.0, 272.7 | Polygalaxanthone V | |
| 15 | 16.45 | 754 | 752.9 | 1507.1 | 705.0, 692.5, 630.8 | Telephiose E |
| 16 | 17.16 | 754 | 752.8 | 1506.9 | 546.7, 223.1 | Sibiricose A4 |
| 17 | 17.71 | 724 | 722.7 | 1447.3 | 696.5, 546.8, 516.6 | Arillanin A |
| 18 | 19.21 | 652 | 650.7 | 528.8, 222.5, 204.5 | Acylsucroses 3′-O-(4-hydroxy-3,5-dimethoxy-E-cinnamoyl), 6-O-benzoyl | |
| 19 | 19.56 | 1484 | 1482.8 | Tenuifoliose Ga | ||
| 20 | 19.79 | 682 | 680.7 | 1362.7 | 442.6, 281.0, 238.9, 136.7 | Tenuifoliside A |
| 21 | 21.85 | 1526 | 1525.4 | 688.7 | Tenuifoliose Fa | |
| 22 | 21.9 | 1496 | 1494.9 | Tenuifoliose La | ||
| 23 | 22.36 | 768 | 766.8 | 1535.8 | 528.8, 237.3, 222.6 | Tenuifoliside C |
| 24 | 22.87 | 1660 | 1659.1 | Polygalasaponin XLVa | ||
| 25 | 23.12 | 1296 | 1294.8 | Tenuifoliose Ca | ||
| 26 | 23.19 | 738 | 736.9 | 1477.5 | 704.8 | Reiniose Aa |
| 27 | 23.94 | 1308 | 1306.8 | 1160.8, 163.3, 145.0 | Tenuifoliose I | |
| 28 | 24.45 | 1338 | 1336.9 | 894.5, 320.6 | Senegose L | |
| 29 | 24.69 | 1296 | 1294.9 | 1118.5, 881.4 | Senegose K | |
| 30 | 25.48 | 1308 | 1306.8 | 964.7 | Reinioside Ba | |
| 31 | 26.07 | 1338 | 1336.8 | Tenuifoliose Ba | ||
| 32 | 26.38 | 1350 | 1348.9 | 1308.4, 1250.1 | Polygalasaponin XXXV | |
| 33 | 26.47 | 1526 | 1525.2 | Tenuifoliose Fa | ||
| 34 | 26.99 | 1380 | 1379.0 | 1336.6, 390.8 | Tenuifoliose A | |
| 35 | 39.91 | 1732 | 1731.2 | 1587.8, 425.4 | Onjisaponin W | |
| 36 | 47.40 | 316 | 314.9 | 214.4, 116.8 | 1,2,3,7-Tetramethoxyxanthone |
— = unknown;
a
= possible structure; MW = molecular weight.