Table 3.
The accuracy of the HPLC-UV method for the determination of peimine and peiminine.
| Analyte | Sample weight (g) | Original (mg) | Spiked (mg) | Found (mg) | Recovery (%) | Mean recovery (%) | RSD (%) |
|---|---|---|---|---|---|---|---|
| Peimine | 0.9755 | 0.4841 | 0.5010 | 0.9827 | 99.53 | 94.18 | 3.75 |
| 0.9702 | 0.4815 | 0.5010 | 0.9332 | 90.17 | |||
| 0.9712 | 0.4820 | 0.5010 | 0.9454 | 92.51 | |||
| 0.9901 | 0.4914 | 0.5010 | 0.9537 | 92.30 | |||
| 0.9961 | 0.4944 | 0.5010 | 0.9620 | 93.34 | |||
| Peiminine | 0.9755 | 0.2915 | 0.3132 | 0.5741 | 90.25 | 91.56 | 2.11 |
| 0.9702 | 0.2899 | 0.3132 | 0.5820 | 93.25 | |||
| 0.9712 | 0.2902 | 0.3132 | 0.5728 | 90.22 | |||
| 0.9901 | 0.2958 | 0.3132 | 0.5808 | 90.99 | |||
| 0.9961 | 0.2976 | 0.3132 | 0.5936 | 94.49 |
HPLC-UV = high performance liquid chromatography-ultraviolet detection; RSD = relative standard deviation.