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HPLC solvent gradient elution program.
| Time (min) | Eluent solvent (%) | |
|---|---|---|
|
| ||
| A | B | |
| 0 | 100 | 0 |
| 5 | 80 | 20 |
| 15 | 70 | 30 |
| 20 | 70 | 30 |
| 30 | 65 | 35 |
| 35 | 65 | 35 |
| 45 | 50 | 50 |
| 55 | 50 | 50 |
| 65 | 30 | 70 |
| 70 | 30 | 70 |
| 75 | 0 | 100 |
| 80 | 0 | 100 |
| 85 | 0 | 100 |
A = 10% methanol and 0.05% formic acid; B = 70% methanol and 0.05% formic acid.
HPLC = high-performance liquid chromatography.
