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Effect of gallic acid (GA) on fluorescence spectra of trypsin (pH =7.4, λex = 280 nm) at different temperatures (A: 25 °C, B: 31 °C, and C: 37 °C), with the trypsin concentration of 4 × 10−5 mol/L, and GA concentration of 0 mol/L, 1.0 × 10−5 mol/L, 2.0 × 10−5 mol/L, 3.0 × 10−5 mol/L, 4.0 × 10−5 mol/L, 5.0 × 10−5 mol/L, 6.0 × 10−5 mol/L, and 8.0 × 10−5 mol/L, respectively (from spectra 1 to spectra 8).
