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Plasmids, bacterial strains, and lambda phages
| Plasmid, bacterial strain, or lambda phage | Genotype or relevant property | Source or reference |
|---|---|---|
| Plasmids | ||
| pT713 | Expression vector | 19 |
| pKA23am | gene2+, gene3 amber | 10 |
| pKA2oc3 | gene2 ochre, gene3+ | 10 |
| pKA23 | gene2+, gene3+ | 10 |
| pGP1-2 | T7 RNApol, ts induction | 19 |
| Strains | ||
| K38 | Host for plasmids | 15 |
| 594 | Su− | 28 |
| 594d(recA-srlR)306::Tn10 | Su−recA | 24 |
| Lambda phages | ||
| Jam | J amber, Int+ | 24 |
| Rts | Rts, Int+ | 24 |
| Jam int4 | J amber, Int− | 24 |
| Rts int4 | Rts, Int− | 24 |
