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. Author manuscript; available in PMC: 2023 Jun 24.
Published in final edited form as: Circ Res. 2022 May 16;131(1):91–105. doi: 10.1161/CIRCRESAHA.122.321050

Figure 4. ATF4 controls the one-carbon metabolic pathway and PPP.

Figure 4.

A. NRVMs were infected by adenoviruses expressing ATF4 or control GFP. RNA-sequencing was conducted and PCA analysis was performed. N=3 for each group.

B. Metabolomics were performed with NRVMs expressing control GFP or ATF4. N=3 for each group.

C. Significantly changed genes (RNA-sequencing) and metabolites (metabolomics) were subjected to the joint pathway analysis (JPA).

D. Multi-omics analysis using transcriptomic and metabolomic data was performed according to the analytical scheme shown in C. JPA was used to calculate the bubble chart, which is a combination of enrichment P values and gene numbers of individual pathways.

E. The top 40 transcripts (left, RNA-sequencing) and 27 metabolites (right, metabolomics) with cutoff threshold of fold change>1.2 or <0.8 and P value adjusted <0.05 are shown. FC, fold change.