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| gene | sequence | length (nt) | product length (nt) | Tm (°C) |
|---|---|---|---|---|
| RUNX2 | TTCGTCAGCGTCCTATCAGTTCC | 23 | 150 | 60.4 |
| CTTCCATCAGCGTCAACACCA | 21 | 60.8 | ||
| Col1a1 | GGACACTACCCTCAAGAGCCTG | 22 | 129 | 61.9 |
| TACTCTCCGCTCTTCCAGTCAGA | 23 | 61 | ||
| OCN | ACAAAGCCTTCATGTCCAAGCA | 22 | 217 | 60.9 |
| GACATGCCCTAAACGGTGGT | 20 | 61.2 | ||
| GAPDH | GGCATCCTGGGCTACACTGA | 20 | 157 | 62.5 |
| CCCTGTTCCTGTAGCCATATTCA | 23 | 62.9 |
