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. 2022 Jul 19;7(30):26812–26823. doi: 10.1021/acsomega.2c03237

Figure 4.

Figure 4

Binding curves of 6-AmHap-mAb against 6-AmHap-Cy5 by ED-fluorimetry. (A) Low hapten-fluorophore tracer concentration: ED of different mAb concentrations against 5 nM 6-AmHap-Cy5. (B) High hapten-fluorophore tracer concentration: ED of different mAb concentrations against 50 nM 6-AmHap-Cy5 (solid red: fluorescence measurement of ED sample/buffer solutions with 50 nM tracer at low laser power; hollow red: fluorescence measurements after a 1:10 dilution of 50 nM ED sample/buffer solutions at high laser power).