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. Author manuscript; available in PMC: 2022 Aug 4.
Published in final edited form as: Cell Rep. 2022 Apr 26;39(4):110740. doi: 10.1016/j.celrep.2022.110740

Figure 4. MBL-O/P regulates its own production by two different mechanisms.

Figure 4.

(A) qRT-PCR of circMbl isoforms in mbl-O/P-KD fly heads.

(B) qRT-PCR of circMbl isoforms fold change in mbl-O/P transgenic fly heads.

(C) qRT-PCR of pre-mbl in MBL-O/P-OE fly heads.

(D) qRT-PCR evaluation of the levels of preRNA in mbl-O/P and Exon1-KD fly heads.

(E) mbl-O/P and preMbl (Ex2-In2) expression levels correlation plot in various mbl isoforms KD flies.

(F) qRT-PCR in mbl-O/P and -C-OE fly heads.

(G) Representation of MBL-C and MBL-O/P regulation in cis by circMbl isoforms in different tissues. In the brain (green), MBL-C binds to pre-mRNA in order to facilitate backsplicing (as described in Ashwal-Fluss et al., 2014). In the eye, MBL-O/P regulates its own levels by two different mechanisms: inhibiting the splicing of the first and second introns (red inhibition symbols) and promoting backsplicing (dashed violet lines).

Tubulin was used as a normalization control (n = 3, error bars represent SEM, two-tailed t test performed for significant difference: ****p < 0.0001, ***p < 0.0002, **p < 0.0021, *p < 0.0332).