KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-MBL | Lab of Prof. Darren Monckton PMID:17309604 | N/A |
| mouse monoclonal Anti-FLAG | Sigma-Aldrich | CAT# F3165; RRID:AB_259529 |
| mouse monoclonal DM1A anti α-tubulin | Sigma-Aldrich | CAT# T6199; RRID: AB_477583 |
| Deposited data | ||
| Ago-IP of circMbl KD and control fly heads | This paper | GEO: GSE118360 |
| 3′RNA seq of KD and OE circMbl fly heads | This paper | GEO: GSE122694 |
| RNAseq from KD of circMbl using UAS-circMbl in combination with different Gal4 | This paper | GEO: GSE122693 |
| Nanopore sequencing from Mbl-exon2 pulldown | This paper | GEO: GSE163780 |
| 3′RNA seq from sh-circMbl Actin-Gal4 KD fly brains | This paper | GEO: GSE163797 |
| Fly brain single cell RNA sequencing | Davie et al. (2018) | GEO: GSE107451 |
| nuclear total RNA-seq libraries prepared from specific cell types using INTACT/TAPIN | Davis et al., (2020) | GEO: GSE116969 |
| Ribosome footprinting data | Pamudurti et al. (2017) | GEO: GSE79626 |
| RNA sequencing | Martin Anduaga et al., 2019 | GEO: GSM3523858 to GSM3523869 (all timepoints at 25C from series GSE124136) |
| Drosophila melanogaster genome and transcriptome build 6, Dm6 | Ensembl | http://ftp.ensembl.org/pub/release-104/fasta/drosophila_melanogaster/dna/ and http://ftp.ensembl.org/pub/release-104/gtf/drosophila_melanogaster |
| Experimental models: Cell lines | ||
| D. melanogaster: Cell line S2: S2-DRSC | N/A | N/A |
| Experimental models: Organisms/strains | ||
| D. melanogaster: w1118 | Bloomington Drosophila stock center | BDSC: 5905 FlyBase:FBal0018186 |
| D. melanogaster: yv1 | Bloomington Drosophila stock center | N/A |
| D. melanogaster: Actin-Gal4: w[1118]; P{w [+mC] = AyGAL4}25/CyO | Bloomington Drosophila stock center | BDSC: 3953 FlyBase:FBti0012290 |
| D. melanogaster: Elav-Gal4 | Bloomington Drosophila stock center | BDSC: 458 FBgn0260400 |
| D. melanogaster: UAS-Dcr2 | Bloomington Drosophila stock center | N/A |
| D. melanogaster: UAS-circMbl-OE | Pamudurti et al. (2017) PMID: 28344080 | N/A |
| D. melanogaster: UAS-MBL-C-FLAG OE | This paper | N/A |
| D. melanogaster: UAS-MBL-O/P-FLAG OE | This paper | N/A |
| D. melanogaster: Scrambled Control (8MM) | This paper | N/A |
| D. melanogaster: MBL-A-KD1 | This paper | N/A |
| D. melanogaster: MBL-A-KD2 | This paper | N/A |
| D. melanogaster: MBL-B-KD1 | This paper | N/A |
| D. melanogaster: MBL-B-KD2 | This paper | N/A |
| D. melanogaster: MBL-C-KD1 | This paper | N/A |
| D. melanogaster: MBL-C-KD2 | This paper | N/A |
| D. melanogaster: MBL-O/P-KD1 | This paper | N/A |
| D. melanogaster: MBL-O/P-KD2 | This paper | N/A |
| D. melanogaster: MBL-UTR-KD1 | This paper | N/A |
| D. melanogaster: MBL-UTR-KD2 | This paper | N/A |
| D. melanogaster: MBL-M/I-KD1 | This paper | N/A |
| D. melanogaster: MBL-M/I-KD2 | This paper | N/A |
| D. melanogaster: MbL-E1-KD1 | This paper | N/A |
| D. melanogaster: MbL-E1-KD2 | This paper | N/A |
| D. melanogaster: MbL-E2-KD1 | This paper | N/A |
| D. melanogaster: MbL-E2-KD2 | This paper | N/A |
| D. melanogaster: MbL-E2–3-KD1 | This paper | N/A |
| D. melanogaster: MbL-E2–3-KD2 | This paper | N/A |
| D. melanogaster: circMbl KD1 | This paper | N/A |
| D. melanogaster: circMbl KD2 | This paper | N/A |
| D. melanogaster: circMbl KD3 | This paper | N/A |
| Oligonucleotides | ||
| Oligos for cloning, and qPCR | This paper | Table S7 |
| Recombinant DNA | ||
| UAS-MBL-C-FLAG OE | This paper | N/A |
| UAS-MBL-O/P-FLAG OE | This paper | N/A |
| Software and algorithms | ||
| tophat | Trapnell et al. (2009) | https://ccb.jhu.edu/software/tophat/index.shtml |
| Samtools | (Li et al., 2009) | http://samtools.sourceforge.net/ |
| SYLAMER algorithm | van Dongen et al. (2008) | https://www.ebi.ac.uk/research/enright/software/sylamer |
| Find_circ | Memczak et al. (2013) | https://github.com/marvin-jens/find_circ |
| DEXseq | Anders et al. (2012) | https://bioconductor.org/packages/release/bioc/html/DEXSeq.html |
| DEseq | https://www.bioconductor.org/packages//2.10/bioc/html/DESeq.html | |
| DEseq2 | Love et al. (2014) | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| ESAT tool | Derr et al. (2016) | https://github.com/garber-lab/ESAT |
| STAR | Dobin et al. (2013) | https://github.com/alexdobin/STAR |
| Seurat R package version 3.0.2 | Butler et al. (2018) | https://satijalab.org/seurat/articles/install.html |
| Minimap2 | (Li, 2018) | https://github.com/lh3/minimap2 |
| Rsubread | (Liao et al., 2019) | https://bioconductor.org/packages/release/bioc/html/Rsubread.html |
| ciRcus | https://github.com/BIMSBbioinfo/ciRcus | |