TABLE 1.
Probes used for Northern blot analysis in this studya
| Probe | Length (bp) and position | Oligonucleotides used to obtain PCR fragments | EMBL/GenBank accession no. |
|---|---|---|---|
| a | 242 (16996–17237) | 5′-GAAGACGAGATGAAAGAGGGG-3′ | AL021899 |
| 5′-GTAAGAATGCCCTTGTCGTAGG-3′ | |||
| b | 238 (16592–16828) | 5′-ATTACTCGCCGCCTATCG-3′ | AL021899 |
| 5′-AGTTGCTGCGGTGTGATCC-3′ | |||
| c | 289 (16127–16415) | 5′-AGATCGTGATTGCCGTGC-3′ | AL021899 |
| 5′-ACCAGCTCCGCCAACACC-3′ | |||
| d | 246 (15782–16028) | 5′-AGGGCTTTGGTGCGGTAGC-3′ | AL021899 |
| 5′-AGGCTGTAAAGATCCAGACCG-3′ | |||
| 16S | 336 (109–446) | 5′-GCCTGGGAAACTGGGTCTAA-3′ | mtu16srn |
| 5′-TCTCCACCTACCGTCAATCC-3′ |
a
Probe a is hspX gene specific. Probes b, c, and d are representative of three parts of the hspX downstream gene. The positions of probes a, b, c, and d correspond to those in Fig. 1A. 16S indicates the probe specific to 16S rRNA gene.