Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 27;32:100823. doi: 10.1016/j.neo.2022.100823

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Authors. Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

SOMCL-19-133 demonstrates potent and selective inhibition of NAE. (a) Chemical structures of MLN4924 and SOMCL-19-133. (b and c) Dose-response curves of enzymatic inhibition by SOMCL-19-133. (b) NAE; (c) UAE. NAE inhibitor MLN4924 and UAE inhibitor MLN7243 were used as the positive control, respectively. Data are represented as mean ± SD from three independent experiments. S133 is short for SOMCL-19-133. (d) Representative western blot analysis showing the effect of SOMCL-19-133 on Ubc12^NEDD8, UBC10^Ub, and Ubc9^SUMO thioester levels. HCT-116 cells were treated with different concentrations of SOMCL-19-133 and MLN4924 for 4 h. Cell lysates were separated by non-reducing SDS-PAGE and immunoblotted using the indicated antibodies. The experiment was repeated three times. (e) Kinase affinity assay of SOMCL-19-133 against 468 kinases and some of their mutants at 1 µM with DiscoveRx KINOMEScan screening platform and presented as a TREEspot interaction map.