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(a, b, c) TRAP staining for 7 d, the number and the area of TRAP-positive cells; (d, e) F-actin for 7 d, DAPI and Merge, statistical analysis; (f) WB: NFATc1, C-FOS, Cath-K, p-IKBα and p-NFκB proteins for 7 d; (g, h, i, j, k) Statistical Analysis. ∗P < 0.05, ∗∗P < 0.01.
