Figure 12.

Rim11 levels during cell growth. Yeast cells expressing TAP-tagged Rim11 were grown for the indicated time intervals in synthetic complete medium. The cell density measured by A₆₀₀ at the time of harvest is also indicated. Cell extracts were prepared, and 25 μg total protein was used for Western blot analysis with anti-protein A and anti-Pgk1 (loading control) antibodies. Top, portions of representative blots from three independent experiments are shown, and the positions of molecular mass standards, Rim11-PtA, and Pgk1 are indicated. Bottom, amounts of Rim11-PtA and Pgk1 were quantified with ImageQuant software, and the relative amount of Rim11-PtA/Pgk1 is presented. The data shown in the figure are the average of three independent experiments ± SD (error bars). The individual data points are also shown.