Table 2.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristics | Source or Reference |
|---|---|---|
| Strain | ||
| S. cerevisiae | ||
| RS453 | MATa ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-52 | (110) |
| SS1026 | pah1Δ::TRP1 derivative of RS453 | (14) |
| SS1132 | pah1Δ::TRP1 nem1Δ::HIS3 derivative of RS453 | (48) |
| W303-1A | MATaade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | (111) |
| BY4741 | MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Thermo Fisher Scientific |
| BY4741-rim11Δ::KanMX | rim11Δ::KanMX of BY4741 | Thermo Fisher Scientific |
| SKY001 | rim11Δ::KanMX derivative of W303-1A | This study |
| BY4741-RIM11-TAP | TAP-tagged RIM11 strain | Thermo Fisher Scientific |
| E. coli | ||
| DH5α | F- φ80 lacZΔΜ15Δ (lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk-mk+) phoA supE44 λ−thi-1 gyrA96 relA1 | (88) |
| BL21(DE3)pLysS | F-ompT hsdSB (rB-mB-) gal dcm (DE3) pLysS | Novagen |
| BL21(DE3) | F-ompT hsdSB (rB-mB-) gal dcm (DE3) | Invitrogen |
| Plasmid | ||
| pET-15b | E. coli expression vector with N-terminal His6-tag fusion | Novagen |
| pGH313 | PAH1 coding sequence inserted into pET-15b | (2) |
| pRS415 | Single-copy number E. coli/yeast shuttle vector with LEU2 | (112) |
| pGH315 | PAH1 inserted into pRS415 | (80) |
| pSK005 | PAH1 (T163A/T164A) derivative of pGH315 | This study |
| pSK006 | PAH1 (S602A) derivative of pGH315 | This study |
| pSK007 | PAH1 (T163A/T164A/S602A) derivative of pGH315 | This study |
| pHC204 | PAH1 (S110A/S114A/S168A/S602A/T723A/S744A/S748A) derivative of pGH315 | (80) |
| pSK003 | RIM11 inserted into pRS415 | This study |
| pYES2 | High-copy number E. coli/yeast shuttle vector with URA3 and the GAL1 promoter | Thermo Fisher Scientific |
| pSK001 | RIM11-TAP in pYES2 | This study |
| EB1164 | PHO85-His6 derivative of pQE-60 | (96) |
| EB1076 | PHO80 derivative of pSBETA | (96) |
| YCplac111-GAL1/10- NEM1-PtA | NEM1-PtA under control of GAL1/10 promoter inserted in CEN/LEU2 plasmid | (14) |
| pRS313-GAL1/10-SPO7 | SPO7 under control of GAL1/10 promoter inserted in CEN/HIS3 plasmid | (24) |