TABLE 1.
Bacterial strains and plasmids
| Species, strain, serogroup, or plasmid | Relevant properties | Reference(s) or source |
|---|---|---|
| Bacteria | ||
| L. pneumophila | ||
| AA100 | 3 | |
| AA400 | AA100; asd::Kanr | 25 |
| BS100 | AA100; pilEL::Kanr | 50 |
| BS200 | AA100; Nalr | This study |
| Serogroup 1 | pilEL+ | 50 |
| Serogroup 2 | pilEL+ | 50 |
| Serogroup 3 | pilEL+ | 50 |
| Serogroup 4 | pilEL+ | 50 |
| Serogroup 5 | pilEL+ | 50 |
| Serogroup 6 | pilEL+ | 50 |
| Serogroup 7 | pilEL+ | 50 |
| Serogroup 8 | pilEL+ | 50 |
| Serogroup 9 | pilEL+ | 50 |
| Serogroup 10 | pilEL+ | 50 |
| Serogroup 11 | pilEL+ | 50 |
| Serogroup 12 | pilEL+ | 50 |
| Serogroup 13 | pilEL+ | 50 |
| L. wadsworthii | pilEL+ | 50 |
| L. santicrucis | pilEL+ | 50 |
| L. cherii | pilEL+ | 50 |
| L. longbeachae | pilEL | 50 |
| L. gormanii | pilEL | 50 |
| L. micdadei | pilEL | 50 |
| E. coli DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 24 |
| Plasmids | ||
| pBC SK+ | ColE1 origin, lacZα, Cmr | Stratagene (La Jolla, Calif.) |
| pBC-K | pBC SK+; Cmr Kanr | This study |
| pUC4K | pBR322 origin; Ampr Kanr; source for the Kanr marker | Pharmacia Biotech (Piscataway, N.J.) |
| pBJ120 | pBC SK+; CmrpilEL+ | 50 |
| pBJ114 | pBC SK+; CmrpilEL::Kanr | 50 |
| pBOC20 | oriV oriT sacB Cmr | 6 |
| pBJ115 | pBOC20; CmrpilEL::Kanr | 50 |
| pGS-K | pBOC20; CmrgspA::Kanr | 4 |
| pJA2A-K2 | pBOC20; Cmrppa::Kanr | 2 |
| pOHBOC1 | pBOC20; Cmrasd+ | 25 |
| pOHBOC1-K | pBOC20; Cmrasd::Kanr | 25 |
| pOHBOC2 | pBOC20; Cmrasd+ | 25 |
| pTLP6 | oriV oriT rpsL cos′ Cmr | 7, 34 |
| pCC3 | pTLP6; CmrpilEL+ | 50 |