TABLE 2.
Effect of culture conditions on transformation frequency
| Plasmid | Culture medium | Temp (°C) | Other culture condition | Transformation frequencya |
|---|---|---|---|---|
| pBC-K | BYE | 37 | <10−9 | |
| pBJ114 | BYE | 37 | 3.9 × 10−7 ± 1.3 × 10−7 | |
| pBC-K | BYE | 30 | <10−9 | |
| pBJ114 | BYE | 30 | 9.5 × 10−6 ± 2.0 × 10−6 | |
| pBC-K | BYE | 24 | <10−9 | |
| pBJ114 | BYE | 24 | 1.6 × 10−7 ± 1.4 × 10−8 | |
| pBC-K | BYE | 37 | Aeration | <10−9 |
| pBJ114 | BYE | 37 | Aeration | <10−9 |
| pBC-K | BYE | 37 | CO2 | <10−9 |
| pBC-K | BCYE | 37 | CO2 | <10−9 |
| pBJ114 | BCYE | 37 | <10−9 | |
| pBC-K | Water | 37 | <10−9 | |
| pBJ114 | Water | 37 | <10−9 | |
| pBC-K | Water | 24 | <10−9 | |
| pBJ114 | Water | 24 | <10−9 |
a
Transformation frequency was measured after addition of 40 μg of plasmid DNA per ml.