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Enzyme kinetic incubation conditions
| Substrate | Enzyme | Incubation time (min) | Protein conc. (µg/µL) | Substrate conc. (µM) |
|---|---|---|---|---|
| Thebacon | hCES1b | 5 | 0.05 | 0–3000 |
| hCES1c | 5 | 0.05 | 0–2000 | |
| hCES2 | 5 | 0.05 | 0–2000 | |
| MMB-CHMICA | hCES1b | 15 | 0.05 | 0–250 |
| MMB-4en-PICA | hCES1c | 30 | 0.1 | 0–2000 |
| MMB-FUBINACA | hCES1b | 15 | 0.05 | 0–500 |
| hCES1c | 10 | 0.05 | 0–1000 | |
| MPhP-5F-PICA | hCES1b | 20 | 0.05 | 0–500 |
| hCES1c | 5 | 0.1 | 0–250 |
