. 2022 Jul 5;96(10):2755–2766. doi: 10.1007/s00204-022-03332-z

Table 3.

Calculated enzyme kinetic parameters (± S.D.) for the ester hydrolysis. K_m, Michaelis–Menten constant; V_max, maximum velocity; Cl_int, in vitro intrinsic clearance

Substrate	Enzyme	K_m (µM)	V_max (AU/min/mg)	Cl_int (AU/min/mg/µM)
Thebacon	hCES1b	655 ± 210	394 ± 40	0.60
	hCES1c	1530 ± 470	831 ± 140	0.54
	hCES2	543 ± 87	482 ± 31	0.89
MMB-CHMICA	hCES1b	39.3 ± 12	20.8 ± 2.2	0.53
MMB-4en-PICA	hCES1c	203 ± 43	7.57 ± 0.42	0.04
MMB-FUBINACA	hCES1b	40.8 ± 15	70.3 ± 7.6	1.7
MMB-FUBINACA	hCES1c	25.7 ± 3.5	30.7 ± 0.96	1.2
MPhP-5F-PICA	hCES1b	88.9 ± 18	182 ± 13	2.0
MPhP-5F-PICA	hCES1c	23.2 ± 4.1	73.6 ± 3.8	3.2