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. 2022 Aug 4;13:4547. doi: 10.1038/s41467-022-32166-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic of the extrachromosomal assay (EC) and pathway-specific probe qPCR (orange primers with blue TMEJ and green NHEJ probes). The microhomology in the TMEJ substrate is shown in red and Polq-dependent synthesis in blue. The helicase-like and polymerase domains of Polθ are shown as yellow ovals, and Ku is shown as red ovals. b TMEJ substrate joining efficiency, normalized with NHEJ substrate joining efficiency to account for differences in transfection efficiency, is expressed as a fraction of the TMEJ observed in wild-type MEFs treated with vehicle (WT DMSO). Data shown are mean ± standard deviation (SD) (n = 3). Statistical significance of differences, relative to WT DMSO cells, was determined by one-way ANOVA (****p < 0.0001; NS, p = 0.87, not significant).