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Effect on processing of mutations in conserved histidine residues in the glutaminase domain and in conserved glutamates in the propeptide. Enzymes with mutations in H25Q (lane 1), H70Y (lane 2), H70N (lane 3), H101Q (lane 4), E2G (lane 5), E-2G/E-1A (lane 6), and the wild type (lane 7) were resolved by electrophoresis on an SDS–8% polyacrylamide gel. Arrows identify the proenzyme and mature enzyme species.
