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. 2022 Aug 4;8:349. doi: 10.1038/s41420-022-01136-9

Fig. 6. CircPOSTN upregulates STC1 and promotes GBM proliferation, migration, and neovascularization.

Fig. 6

A The expression association between STC1 and VEGFA was analyzed based on the TCGA GBM cohort. B The expression levels of STC1 and VEGFA were obtained from the TCGA GBM cohort. C–E The VEGFA levels from the culture medium of U87 cells were detected by ELISA. U87 cells were transfected with circPOSTN overexpressing vectors (C), miR-219a-2-3p inhibitors (D), STC1 overexpressing vectors (E), or the corresponding controls. F–H The U87 cells were transfected with circPOSTN overexpressing or empty control vectors. Then, the HUVECs were cultured in the conditioned medium from U87 cells incubated with anti-VEGF or anti-IgG antibodies. F The MTT measurement of cell proliferation. G The transwell migration assays were conducted to determine the cell migration capacity. H The tubule formation assays showing the pro-angiogenic effects of U87 cells. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. NS not significant.