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. 2022 Aug 4;13:4545. doi: 10.1038/s41467-022-32142-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Anatomical projections from ACC to M2 were visualized using a genetically modified rabies virus system. One to two weeks after helper virus injection in M2 (a cocktail solution of AAV1-synP-FLEX-sTpEpB and pENN.AAV.CaMKII.0.4.Cre.SV40), rabies virus (RVΔG-4mCherry) was injected at the same coordinate. b A coronal section of the virus injection site in M2 (this is a magnified view of a region pointed by the red arrow in panel no. 4 in Supplementary Fig. 8a). Neurons infected by helper virus expressed GFP (coded in green in the image). Scale bar, 0.5 mm. c ACC neurons that were retrogradely infected with rabies virus expressed mCherry (coded in red in the image). Scale bar, 0.5 mm. d Projections from ACC to M2 were validated using AAVretro virus. Sagittal view of rat brain. Rats were injected with AAVretro-pmSyn1-EBFP-cre and AAV5-hSyn-DIO-hM4Di-mCherry viruses in M2 and ACC, respectively. e ACC neurons that were infected with AAVretro virus expressed mCherry (coded in red in the image) after Cre recombination. Scale bar, 0.5 mm.