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. 2022 Aug 4;13:4545. doi: 10.1038/s41467-022-32142-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Histological section for halorhodopsin (eNpHR3.0) expression in ACC. Green, eNpHR3.0-eYFP expression. Blue, DAPI. White dotted line shows reconstructed positions of fiberoptic implants. Scale bar, 500 μm. b Suppression of spiking activity by 561 nm light delivery. Top left, raster plot of a representative single-unit measured in ACC showing spiking activities before, during, and after laser light delivery. Top right, example waveforms of the representative single-unit. Bottom left, peri-event time histogram sorted by the timing of light onset. Bin width, 50 ms. c Optogenetic silencing of ACC after animal’s incorrect 2nd choices (i.e., 2nd choice omission errors). Light was delivered for 4 s after animals pushing the center lever instead of correctly poking the side port opposite to the 1st choice. d Optogenetic silencing of ACC after animal’s correct 2nd choices. Light was delivered for 4 s after a correct 2nd choice (i.e., animals poking the side port opposite to the 1st choice). Source data are provided as a Source Data file.