FIG. 2.

Plate assay for alginate lyase activity. Cells were restreaked on LB plates containing alginate and assayed for alginate lyase activity (see Materials and Methods). 1, untransformed E. coli DH5α cells; 2, E. coli DH5α cells containing pAPL4.7 plasmid; 3, E. coli cells containing pBluescript SK(+) plasmid.