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SDS-PAGE analysis of the recombinant A. chroococcum AlgL protein. (A) Gel stained with Coomassie brilliant blue R-250; (B) alginate lyase activity detected on a gel containing 1% alginic acid. Lanes: M, molecular mass markers; 1, periplasmic fraction (10 μg); 2, hydroxyapatite column AlgL fraction (10 μg); 3, phenyl-Sepharose AlgL fraction (10 μg).
