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Purification of recombinant alginate lyase from A. chroococcum ATCC 4412a
| Step | Total amt of protein (mg) | Total activity (U) | Sp act (U/mg of protein) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Periplasmic fraction | 69.35 | 641.7 | 9.25 | 100 | 1 |
| Hydroxyapatite | 5.6 | 331.8 | 59.25 | 51.7 | 6.4 |
| Phenyl-Sepharose | 0.36 | 33.8 | 93.88 | 5.26 | 10.14 |
The enzyme was purified from 5 g (fresh mass) of E. coli BL-21 (DE3) containing plasmid pAPET-2.
