TABLE 2.
Cation added | Alginate lyase activity (A548) |
---|---|
None | 0.010 |
K+ | 0.173 |
Na+ | 0.014 |
Mg2+ | 0.017 |
K+ plus Mg2+ | 0.927 |
Mg2+ plus Na+ | 0.018 |
K+ plus Mg2+ plus Na+ | 1.059 |
Aliquots of a purified A. chroococcum alginate lyase preparation (30 mU) were assayed for enzyme activity in the presence of the indicated cations at 100 mM (final concentration) each. Other experimental conditions were as in the thiobarbituric acid assay, except that 10 mM Tris-HCl buffer (pH 7.5) was used.