Figure 6.
Effects of AAV9 vectors carrying miR-214-3p TuD or miR-34a-5p in healthy mice
(A) 10-week-old mice were i.v. injected with a single dose of rAAV9 (5 × 1013 kg/vg) carrying ctrl, miR-214-3p TuD, or miR-34a-5p and, 8 weeks later, expression of miR-214-3p (left) or miR-34a-5p (right) in the indicated tissues was assessed by RT-PCR (n = 5). (B–E) Femoral bone mass of rAAV-treated mice was assessed by microCT, demonstrating that the AAV treatment had little to no effect on normal bone homeostasis. Representative images of 3D reconstruction (B and D) and relative quantification (C and E) are displayed (n = 5). (F) Representative images of H&E-stained longitudinal sections of the indicated tissues of AAV-treated mice are displayed (n = 5). Scale bars, 400 μm in (B and D) and 100 μm in (F). Values represent mean ± SD: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 by an unpaired two-tailed Student’s t test (A, B, and D).