Table 1.
Effects of BMS on survival rate in C. elegans.
| Strains | BMS (μg/mL) |
No. Animals |
Median Lifespan (days) |
Mean lifespan (days±SE)a | MaximumLifespan (days) | P-valueb |
|---|---|---|---|---|---|---|
| N2 | 0 | 126 | 18 | 17.07 ± 0.35 | 24 | ＜0.001 |
| 100 | 108 | 20 | 19.91 ± 0.36 | 26 | ||
| 200 | 138 | 20 | 19.43 ± 0.35 | 26 | ＜0.001 | |
| daf-16 | 0 | 160 | 8 | 8.81± 0.3 | 18 | |
| 100 | 144 | 6 | 7.13± 0.2 | 18 | <0.0001 | |
| hlh-30 | 0 | 160 | 12 | 11.71±0.46 | 26 | |
| 100 | 129 | 12 | 12.14±0.47 | 26 | 0.8026 | |
| hlh-30 (RNAi) | 0 | 110 | 20 | 18.02±0.5 | 26 | |
| 100 | 109 | 14 | 14.99±0.57 | 26 | 0.0009 |
Worms were treated with BMS from the late L4 stage. FUDR (120 μM) was added to sterilize the worms during the treatment period. Survivals were recorded every other day until all of the worms died.
a The mean lifespan was referred to the exact time when the survival rate reached to 50%, which was generated by the OASIS application.
b P-values were analyzed by log-rank (Mantel-Cox method) tests. Significant differences were defined at p< 0.05.