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. 2022 Aug 4;13(8):675. doi: 10.1038/s41419-022-05129-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A The typical morphological images of primary CAFs and normal ovarian fibroblasts (NOFs). Bar = 100 μm. B, C Western blotting and RT-qPCR analysis presented the expression of their representative markers (α-SMA, FAP, and vimentin). D CCK8 assay was used to analyze the viability of SKOV3 and A2780 cells co-cultured with CAF-CM and NOF-CM. E, F Cell invasion was assessed by Transwell assay in SKOV3 and A2780 cells after 48-h co-cultured with conditioned medium (100×magnification). G, H Cell migration ability was measured by wound healing assay in SKOV3 and A2780 cells (100×magnification). The quantitative analyses were performed using ImageJ software. Results are presented as the mean ± SD of three independent experiments. *P < 0.05, ***P < 0.001, ns not significant.