TABLE 2.
PHB synthase, β-ketothiolase, and acetoacetyl-CoA reductase activities in E. coli harboring various plasmids
Plasmid | PHB synthase (mU/g) | β-Ketothiolase (U/g) | Acetoacetyl-CoA reductase (U/mg) |
---|---|---|---|
pBluescript SK− | NDa | 12.5 | 0.01 |
pBHR68 | 63 | 241 | 0.32 |
pBHR68(S260A) | 0.5 | 205 | 0.3 |
pBHR68(S546I) | 2.3 | 123 | 0.2 |
ND, not detectable.