Table 1. Evaluation of different rNluc-fused Toxoplasma gondii antigens in Luciferase-linked Antibody Capture Assay (LACA) in comparison with enzyme-linked immunosorbent assay (ELISA).
| No. tested | No. positive (%) |
||||||
|---|---|---|---|---|---|---|---|
| LACA |
Commercial ELISA | ||||||
| GRA6 | GRA7 | GRA8 | BAG1 | Cocktail | |||
| Toxoplasma gondii | 10 | 7 (70.0) | 8 (80.0) | 8 (80.0) | 3 (30.0) | 9 (90.0) | 3 (30.0) |
| Toxocara cati | 7 | 1 (14.3) | 0 (0.0) | 2 (28.6) | 7 (100.0) | 0 (0.0) | 0 (0.0) |
| Ascaris suum | 9 | 1 (14.3) | 3 (33.3) | 2 (22.2) | 4 (44.4) | 0 (0.0) | 0 (0.0) |
| Uninfected | 38 | 5 (13.2) | 7 (18.4) | 10 (26.3) | 16 (42.1) | 2 (5.3) | 1 (2.6) |
| Cut-off by ROC (S/N ratio) | 1.161 | 1.142 | 1.128 | 1.03 | 1.412 | 0.150 * | |
| AUC ± SEM | 0.83 ± 0.07 | 0.87 ± 0.05 | 0.78 ± 0.08 | 0.57 ± 0.09 | 0.98 ± 0.02 | 0.89 ± 0.06 | |
| P value | <0.01 | <0.01 | <0.01 | 0.47 | <0.01 | <0.01 | |
| Sensitivity (%) | 70.0 | 80.0 | 80.0 | 30.0 | 90.0 | 30.0 | |
| Specificity (%) | 87.0 | 81.5 | 74.1 | 50.0 | 96.3 | 98.2 | |
| Accuracy (%) | 84.4 | 81.3 | 75.0 | 39.1 | 95.3 | 87.5 | |
AUC, area under the receiver operating characteristic (ROC) curve; SEM, standard error; asterisk (*) indicated the cut-off value (OD) recommended by the manufacturer.