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. 2022 May 18;84(7):905–913. doi: 10.1292/jvms.22-0099

Table 2. Kappa coefficient values of different rNluc-fused Toxoplasma gondii antigens in Luciferase-linked Antibody Capture Assay (LACA) compared to Dye-Test as “gold standard” using pig serum samples.

Dye-Test
Kappa value 95% CI
Positive Negative
GRA6-LACA Positive 13 25 0.230 0.044 to 0.417
Negative 8 53

GRA7-LACA Positive 10 7 0.415 0.192 to 0.638
Negative 11 71

GRA8-LACA Positive 17 24 0.372 0.199 to 0.545
Negative 4 54

BAG1-LACA Positive 4 11 0.055 –0.151 to 0.261
Negative 17 67

Cocktail LACA Positive 21 7 0.811 0.679 to 0.944
Negative 0 71

Commercial ELISA Positive 2 6 0.024 –0.155 to 0.203
Negative 19 72

Total 21 78

CI, confidence interval.