TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Reference or source | Comment(s) |
|---|---|---|
| S. aureus | ||
| ISP3 | J. Iandolo | Strain used for genomic sequencing |
| 8325-4 | 20 | NCTC 8325 cured of prophages, plasmid free |
| S. epidermidis | ||
| 35983 | ATCCa | Blood isolate from patient with intravascular catheter |
| 35984 | ATCC | Catheter sepsis isolate |
| 49134 | ATCC | Clinical isolate |
| E. coli | ||
| H1717 | 9 | aroB fhuF::λplacMu |
| XL1-Blue | Stratagene | Highly transformable strain |
| M15(pREP4) | Qiagen | Host strain for pQE10 |
| H1717(pFur7,8) | This study | E. coli H1717 carrying pFur7,8 |
| H1717(pFur9,10) | This study | E. coli H1717 carrying pFur9,10 |
| H1717(pFur11,12) | This study | E. coli H1717 carrying pFur11,12 |
| Plasmids | ||
| pUC18 | 31 | E. coli cloning vector |
| pQE10 | Qiagen | E. coli expression vector |
| pFur7,8 | This study | pUC18 containing E. coli Fur1 sequence (26) (complementary oligonucleotides Fur 7 and Fur 8) |
| pFur9,10 | This study | pUC18 containing S. aureus sirA Fur-like sequence (complementary oligonucleotides Fur 9 and Fur 10) |
| pFur11,12 | This study | pUC18 containing scrambled sirA Fur-like sequence (complementary oligonucleotides Fur 11 and Fur 12) |
a
ATCC, American Type Culture Collection.