Table 1.
Composition of the basal experimental diet for red sea bream (Pagrus major).
| Ingredients | Percentage (%) | Feed proximate composition | Percentage (%) |
|---|---|---|---|
| Fish meal | 30.00 | Moisture | 5.02 |
| Wheat flour | 28.88 | Crude protein | 44.02 |
| Chicken by product | 31.20 | Crude lipid | 13.78 |
| Fish oil | 4.00 | Crude ash | 12.61 |
| Squid liver powder | 3.00 | ||
| Lecithin | 1.00 | Supplement for BSD group | |
| Mono calcium phosphate | 0.20 | Bacillus sp. PM8313 | 1 × 108 CFU/g |
| Vitamin C | 0.50 | ||
| Vitamin premixa | 0.50 | Supplements for BGSD group | |
| Mineral premixb | 0.50 | Bacillus sp. PM8313 | 1 × 108 CFU/g |
| Cholinec | 0.12 | β-glucan | 0.10% |
| Cellulose | 0.10 | ||
aVitamin premix (as mg kg−1 in diets): Ascorbic acid, 300; dl-Calcium pantothenate, 150; Choline bitate, 3000; Inositol, 150; Menadion, 6; Niacin, 150; Pyridoxine. HCl, 15; Rivoflavin, 30; Thiamine mononitrate, 15; dl-α-Tocopherol acetate, 201; Retinyl acetate, 6; Biotin, 1.5; Folic acid, 5.4; Cobalamin, 0.06.
bMineral premix (as mg kg−1 in diets): NaCl, 437.4; MgSO4·7H2O, 1379.8; ZnSO4·7H2O, 226.4; Fe-Citrate, 299; MnSO4, 0.016; FeSO4, 0.0378; CuSO4, 0.00033; Ca(IO)3, 0.0006; MgO, 0.00135; NaSeO3, 0.00025.