Table 3.

Clonality assessment methods

Assessment method
For CTCL, T-cell clonality can be established by one of the following molecular methods
BIOMED-2 or other PCR-based, approved protocol for both  TCR-γ and TCR-β gene rearrangement
Next generation sequencing (high-throughput sequencing)
For CBCL, B-cell clonality can be established by one of the following molecular methods
BIOMED-2 protocol or other PCR-approved method for  immunoglobulin heavy chain (IgH) and/or Igκ  rearrangement
Surface or cytoplasmic light chains restriction by flow cytometry  (peripheral blood or tissue samples)
Monotypic surface light chains restriction by  immunohistochemistry (κ and λ immunostain)
Light chains restriction by conventional or RNA-scope  ultrasensitive in situ hybridization
IgH rearrangement by next generation sequencing for IgH or  Igκ