Table 2.
β-lactam and aminoglycoside resistance determinants and their context.
| Isolate No. | Resistance to β-lactams | Resistance to aminoglycosides | ||
|---|---|---|---|---|
| Intrinsic resistance genes (An upstream ISAba1) | Acquired resistance genes (associated MGEs) | Intrinsic resistance genes | Acquired resistance genes (associated MGEs) | |
| M01 |
blaADC−30 (present) blaOXA−66 (ND) |
blaOXA−23 (Tn2006) blaTEM−1 (AbGRI2-15) |
ant(3”)-II |
aacA4 (AbGRI3-1) aadA1 (AbGRI3-1) aphA1 (AbGRI2-15) armA (AbGRI3-1) strA (AbGRI1-like-1) strB (AbGRI1-like-1) |
| M02 |
blaADC (interrupted by IS1008) blaOXA−94 (present) |
blaNDM−1 (ISAba14 bracketed transposon also carrying aphA6) |
aadB (pRAY*, JQ904627.1) aphA6 (ISAba14 bracketed transposon also carrying blaNDM−1) |
|
| M03 |
blaADC−57 (ND) blaOXA−64 (ND) |
blaOXA−23 (AbaR4) blaPER−7 (RI-PER-7, in an undetermined plasmid closely similar to pPM194229_1) |
aphA6 (ND) armA (RI-PER-7, in a plasmid closely similar to pPM194229_1) strA (plasmid closely similar to pPM194229_1) strB (plasmid closely similar to pPM194229_1) |
|
| M04 |
blaADC−73 (present) blaOXA−66 (absent) |
blaOXA−23 (AbaR4b) blaTEM−1 (AbGRI2-15) |
aphA1 (AbGRI2-15) armA (AbGRI3-1) strA (AbaR4b) strB (AbaR4b) |
|
| M05 |
blaADC−73 (ND) blaOXA−66 (absent) |
blaOXA−23 (AbaR4b) blaTEM−1 (AbGRI2-15) |
aphA1 (AbGRI2-15) aphA6 (RepAci6 plasmid 99.9% similar to pACICU2) armA (AbGRI3-1) strA (AbaR4b) strB (AbaR4b) |
|
| M06 |
blaADC−79 (present) blaOXA−69 (ND) |
None | aadB (pRAY*-like,99.98% similarity) | |
| M09 |
blaADC (ND) blaOXA−69 (ND) |
blaOXA−23 (Tn2006) | aadB (pRAY*, JQ904627.1) | |
| M10 |
blaADC (ND) blaOXA (ND) |
blaNDM−1 (ISAba14 bracketed transposon also carrying aphA6) blaCARB−16 (ND) |
aadB (pRAY*-V1, JF343536.2) aphA6 (ISAba14 bracketed transposon also carrying blaNDM−1) |
|
| M11 |
blaADC (interrupted by IS1008) blaOXA−94 (present) |
blaNDM−1 (ISAba14 bracketed transposon also carrying aphA6) |
aadB (pRAY*, JQ904627.1) aphA6 (ISAba14 bracketed transposon also carrying blaNDM−1) |
|
| M12 | blaADC−79 (present) | blaOXA−23 (Tn2006) | None | |
| blaOXA−69 (absent) | ||||
| M13 | blaADC−73 (present) |
blaOXA−23 (AbaR4b) blaTEM−1 (AbGRI2-15) |
aphA1 (AbGRI2-15) armA (AbGRI3-1) strA (AbaR4b) strB (AbaR4b) |
|
| blaOXA−66 (ND) | ||||
| M14 |
blaADC−57 (ND) blaOXA−51−like (ND) |
blaOXA−23 (AbGRI1-like-2) |
strA (AbGRI1-like-2) strB (AbGRI1-like-2) |
|
| M15 |
blaADC−79 (present) blaOXA−69 (absent) |
blaOXA−23 (Tn2006) | None | |
| M16 |
blaADC−30 (present) blaOXA−66 (absent) |
blaOXA−23 (Tn2006) blaTEM−1 (AbGRI2-15) |
aacA4 (AbGRI3-1) aadA1 (AbGRI3-1) aphA1 (AbGRI2-15) armA (AbGRI3-1) strA (AbGRI1-like-1) strB (AbGRI1-like-1) |
|
| M17 |
blaADC−30 (present) blaOXA−66 (absent) |
blaOXA−23 (Tn2006) blaTEM−1 (AbGRI2-15) |
aacA4 (AbGRI3-1) aadA1 (AbGRI3-1) aphA1 (AbGRI2-15) |
|
|
armA (AbGRI3-1) strA (AbGRI1-like-1) strB (AbGRI1-like-1) |
||||
| M18 |
blaADC−258a (absent) blaOXA−94 (absent) |
blaNDM−1 (ISAba14 interrupted Tn125) | aadB (pRAY*, JQ904627.1) | |
| M19 |
blaADC−257 (absent) blaOXA−91 (absent) |
blaNDM−1 (ISAba14 interrupted Tn125) blaCARB−16 (ND) |
aadB (pRAY*-V1, JF343536.2) aphA6 (ISAba14-aphA6-ISAba125) |
|
| M20 |
blaADC−73 (absent) blaOXA−66 (absent) |
blaNDM−1 (ISAba14 interrupted Tn125) blaTEM−1 (AbGRI2-15) blaOXA−23 (Tn2006) |
aphA1 (AbGRI2-15) aphA6 (ISAba14-aphA6-ISAba125) aadA1(AbGRI3-4) aacA4 (AbGRI3-4) armA (AbGRI3-4) |
|
anovel variant; ND, could not be determined; MGEs, mobile genetic elements. The symbol * is part of the plasmid's name.