FIGURE 2.

Evaluation of 36 cleaning in place conditions, implemented by applying up to three different cleaning agents in three sequential steps, each deployed at the end of a purification run to generate the chromatography flowthrough product pool (ChromP) from clarified cell culture harvest (CCCH) using RoboColumns: (A) Total signals from optical absorbance measurements at 280 nm from fractions collected during the loading, washing, application of cleaning in place steps 1–3, and post‐CIP flushing the columns (FT, Wash, CIP1, CIP2, CIP3, and PCIP, respectively) per cleaning condition; (B–H) SDS‐PAGE analysis of resin extracts collected at the end of each CCCH purification and deployment of the cleaning conditions. In (B–H), boxes above each gel denote the samples tested per lane and gel. In these boxes, numbers correspond to cleaning conditions, also shown on the x‐axis of (A), and asterisks denote replicated experiments using previously tested cleaning conditions. In (D), the underlined condition 23 is a spurious result due to an error in loading the gel